WebI am interested in understanding the role of the NURF complex and more specifically the BPTF gene in different types of cancer. Graduate Teaching Assistant WebSep 23, 2014 · The NURF complex is composed of the chromatin remodelling ATPase ISWI and the components NURF-38, NURF-301 and CAF-1/p55 [21]; for review see [22]. This complex is well conserved and is the founding member of several ISWI containing remodelling complexes.
The emerging role of ISWI chromatin remodeling complexes in cancer
WebOct 6, 2015 · ShRNA-mediated silencing of the NURF subunit BPTF revealed its essential role in several melanoma cell lines and in untransformed melanocytes in vitro. Comparative … WebMar 28, 2024 · Silencing of the NURF complex subunit SNF2L, BPTF or RBBP4 similarly attenuated GC cell growth and increased apoptosis. circ-DONSON knockdown inhibited GC growth in vivo. Conclusion circ-DONSON promotes GC progression through recruiting the NURF complex to initiate SOX4 expression. Background farm fresh ipo price now
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WebDec 2, 2024 · The immune complex was eluted in elution buffer (1% SDS, 0.1 M NaHCO 3) for 30 min at RT. The eluates were collected and incubated with 10 μl of 5M NaCl to reverse histone-DNA crosslinks at 65°C for 4 h, followed by incubation with proteinase K at 45°C for 1 hr to remove proteins. The remaining DNA was purified with Nucleospin PCR clean up ... WebAug 28, 2009 · The NURF complex is required for the transcriptional activation of some Wg targets in Kc cells. Cells were treated with the indicated dsRNAs for 4 days and total RNA were isolated for RT–PCR. The messenger RNA levels of actin, the ribosome protein L49 and TATA binding protein (TBP) were used as internal controls for normalization. WebJan 6, 1998 · The Drosophila nucleosome remodeling factor (NURF) is a protein complex of four distinct subunits that assists transcription factor-mediated chromatin remodeling. … farm fresh ipo review