Tīmeklis2004. gada 10. nov. · In the pGAL1-Δntcdc6-rereplicating strain, however, Rad53p hyperphosphorylation was detected within 45 min of induction, and the majority of the protein became hyperphosphorylated by 120 min. Together, the metaphase arrest and Rad53p hyperphosphorylation indicate that Rad53p is activated as part of a … TīmeklisRad53p and Dun1p are known to be responsible for 50% of the arrest observed incdc13-1 mutants (Gardner et al. 1999). We suggest that protein “P” is then …

ORC and the intra-S-phase checkpoint: a threshold regulates …

TīmeklisSoluble Rad53p was then transferred and assayed for kinase activity to prevent Mec1p kinase from interfering with detection of Rad53 kinase activity. Consistently, Rad53 … Tīmeklis2002. gada 26. jūl. · Consistent with this idea, Mec1p-dependent activation of Rad53p requires interaction of the latter with the Rad9p adaptor protein, after Rad9p has itself been phosphorylated by Mec1p (31, 32). This inducible interaction is mediated by the FHA domains of Rad53p, which recognize phosphorylated threonine residues in … clock systohc

抗Rad9S1129酵母_价格-品牌-详情介绍_丁香通

TīmeklisDNA stress-triggered induction of RNR3 transcription via the DNA damage checkpoint pathway. Upon DNA damage or abnormally stalled DNA chain elongation, the Mec1p … TīmeklisThe regulation of c-Jun transcriptional activity by Jun N-terminal kinase (JNK) has become a paradigm for understanding how mitogen-activated protein (MAP) kinase signalling pathways elicit specific changes in gene transcription through selective phosphorylation of nuclear transcription factors. Selective phosphorylation of c-Jun … TīmeklisWe have established a new protein-engineering strategy termed “directed domain-interface evolution” that generates a binding site by linking two protein domains and then optimizing the interface between them. Using this strategy, we have generated synthetic two-domain “affinity clamps” using PDZ and fibronectin type III (FN3) domains as the … clock tabcountdowntimer tabmsg tab