site stats

Phenotypes of wehi 231

Web10. jan 2003 · WEHI-231 and Bal 17 B cell lines are representative models for immature and mature B cells, respectively. Their regulation of cytosolic Ca 2+ concentration ([Ca 2+] c) was compared using fura-2 fluorescence ratiometry.The ligation of B cell antigen receptor (BCR) by anti-IgM antibody induced a slow but large increase of [Ca 2+] c in WEHI-231 cells … Web1. jún 1985 · In order to study the mechanism of growth inhibition, we have mutagenized WEHI-231 with ethylmethane sulfonate and cloned the surviving colonies in the presence …

Growth regulation of the B lymphoma cell line WEHI-231 by anti

Web14. máj 2004 · The initial goal of the present study was to elucidate the effects of 2-APB on membrane currents, which revealed the presence of novel K(+) channels in WEHI-231 cells. Under whole-cell patch clamp conditions, 2-APB induced background K(+) current (I(K,bg)) and hyperpolarization in WEHI-231 cells. WebPhenotype of WEHI-231 cells. WEHI-231 cells were stained with Ghost-Red (viability marker) and the following antibodies to determine their phenotype: IgM, CD43, CD23, CD19, and CD93. run program when windows starts https://tywrites.com

The regulation of growth and differentiation of a murine B cell ...

WebWEHI-231 cells undergo apoptosis in response to antigen receptor cross-linking with anti-Ig reagents. Death is specifically triggered via surface IgM (sIgM); cross-linking sIgD, Ia or … Web8. máj 2007 · WEHI-231 cells were chosen as a representative leukocyte cell line with high p110δ expression, whereas NIH 3T3 cells are similar to mouse embryo fibroblasts, a cell … WebWEHI-231/mycER cells 3. Induction of MIG-1 expression by mycER 4. Sequence of 1678 bp cDNA clone of MIG-1 isolated from WEHI-231 cDNA library 5. Northern blot analysis of MIG-1 mRNA expression 6. Lack of induction of MIG-1 in antigen receptor-stimulated WEHI-231 cells 7. Effect of antigen receptor stimulation on MIG-1 expression in 2PK3 and BAL ... run programs in cmd

Roles of the tumor suppressor p53 and the cyclin-dependent …

Category:Differentiation and Ig-Allele Switch in Cell Line WEHI-2311

Tags:Phenotypes of wehi 231

Phenotypes of wehi 231

小鼠血细胞WEHI3细胞_价格-品牌-详情介绍_丁香通

WebIn the present study, we analyzed two variants of the WEHI-231 cell line (Ml and M22) that were selected for growth in the presence of anti-IgM antibodies (32) and demonstrated … WebUsing WEHI 231 cells as a model, we investigated the effects of dexamethasone, cAMP, selected growth factors/ cytokines, DNA damaging agents, metabolic inhibitors and lipid …

Phenotypes of wehi 231

Did you know?

Web25. mar 1991 · Stimulation of the antigen receptor of WEHI-231 B lymphoma cells with anti-receptor antibodies (anti-IgM) induces irreversible growth arrest. Anti-IgM stimulates two kinds of transmembrane signaling events, phosphorylation of proteins on tyrosyl residues and breakdown of inositol phospholipids, which results in increases of inositol … Web18. máj 1998 · Treatment of WEHI 231 immature B lymphoma cells with an antibody against their surface immunoglobulin M (anti-IgM) induces apoptosis and has been studied …

WebA, cell lysates from WEHI-231, WEHI-231- M2, WEHI-231-M2P1, and WEHI-231-M2P2 were incubated with GST-PAK/glutathione-agarose beads for 1 h prior to pull-down assays. WebEvidence for Unique Calmodulin-Dependent Nuclear Factor-kappa B Regulation in WEHI-231 B Cells . × Close Log In. Log in with Facebook Log in with Google. or. Email. Password. Remember me on this computer. or reset password. Enter the email address you signed up with and we'll email you a reset link. ...

WebIn WEHI 231 clones ectopically expressing c-Myc, apoptosis induced by treatment with TPCK or anti-Ig was significantly diminished and cells continued to proliferate. … Web1. okt 1986 · The growth of WEHI-231, a murine immature B lymphoma cell line, was inhibited by anti-IgM antibodies. The inhibition of proliferation, as measured by …

WebWEHI-231 cells undergo apoptosis in response to antigen receptor cross-linking with anti-Ig reagents. Death is specifically triggered via surface IgM (sIgM); cross-linking sIgD, Ia or FcR has no effect. Apoptosis is preceded by growth arrest in the G o /G 1 phase of the cell cycle and may not occur in all currently available WEHI-231 sublines ...

Web15. nov 2007 · Because of its susceptibility to apoptosis upon Ag receptor cross-linking and lack of IgD expression, cells of the mouse cell line WEHI-231 have been classified as immature B cells. In this study we show that early freezings of the WEHI-231 line express IgD but not CD93, which classifies the cells as more similar to mature B cells. run program with batch fileWeb简单，可重复性高 对小鼠血细胞,wehi-3细胞有毒副作用 转染时需除血清: 阳离子脂质体法: 带正电的脂质体与核酸带负电的磷酸基团形成复合物被细胞内吞: 稳定转染，瞬时转染: 适用性广，转染效率高，重复性好，转染时需除血清。转染效果随细胞类型变化大 ... run program when startupWeb13. apr 2024 · The clinical phenotypes of abnormal oocyte cytoplasmic maturation often manifest in different forms, including fertilization failure, zygotic cleavage failure, and early embryonic arrest . ... Reprod.27, 222–231 (2012). Crossref. PubMed. Google Scholar. 87. run project on dockerWebWEHI-231 is a representative mouse B cell line and under-goes apoptosis by antigenic stimulation, which is a key feature of immature B cells. Experimentally, B cell stimulation is achieved by cross-linking membranous IgM-type B cell recep-tors (BCR ligation) using anti-IgM antibody ( IgM). More- run project with yarnWeb12. jan 2007 · Here, we describe the non-redundant roles of caspase-activated DNase (CAD) and DNaseγ during apoptosis in the immature B-cell line WEHI-231. These cells induce DNA-ladder formation and nuclear ... scdss addresssc dss casesWebB, WEHI-231, WEHI-231-M2, WEHI-bcl-XL, and WEHI-bcl-XL-M2 cells were cultured in triplicated independent flasks in the presence or absence of anti-IgM. Cells were sampled and counted at 0, 24, 48 ... scd specifications