Witryna14 wrz 2024 · It is an established fact that orexin plays an important role in regulating the reproductive axis and the secretions of gonadotropin-releasing hormone (GnRH)/luteinizing hormone (LH). ... respectively) and Testosterone [125 I] RIA (sensitivity, intra- and inter-assay coefficients of variation of the kit were 0.04 ng/ml, … In order to test the MRM method, a pool of CSF samples from patients (n = 20) with various neurological disorders but not NT1 was used (RIA concentration of orexin A>200 pg/mL). CSF was collected in polypropylene tubes under standardized conditions. Each CSF sample was sent to the laboratory within … Zobacz więcej Human orexin-A standard (pyroEPLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2) was purchased from Phoenix Scientific and … Zobacz więcej Analyses were performed on a 1290 HPLC system (Agilent technologies) coupled to a 6490 triple quadrupole mass spectrometer with an Electro Spray Ionisation (ESI) source operating in the positive mode … Zobacz więcej 500 μL of CSF (corresponding on average to 350 μg of proteins) were acidified with orthophosphoric acid (ref. 452289-50ML, Sigma-Aldrich) … Zobacz więcej Calibration curves were generate over a 0–200 pg/mL range (0, 4.0, 7.5, 10.0, 14.0, 19.0, 25.0, 50.0, 100.0, 200.0 pg/mL) in water/acetonitrile/formic acid (80/20/0.1, v-v:v) with unlabeled Orexin-A and spiked with SIS (50 … Zobacz więcej

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Witryna25 maj 2024 · While RIA is considered to be the standard for quantifying orexin in cerebrospinal fluid, the accuracy of RIA or ELISA remains a controversial topic (50–52). More importantly, previous studies suggested that RIA and ELISA might recognize orexin-A breakdown products, even hydrolyzed peptides that have little in common … Witryna2 kwi 2013 · Orexin levels were assayed with radioimmunoassay (RIA). Mann-Whitney test and Kruskal-Wallis test were used to detect differences across groups, and logistic regression was used to explore the association between orexin levels and characteristics of demographic and drug abuse. maybury properties

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Witryna29 sty 2009 · Introduction. Orexin A and B, recently identified in the rat hypothalamus (Sakurai et al., 1998) are endogenous neuropeptide agonists for the G-protein coupled orexin-1 (OX1) and orexin-2 (OX2) receptors (Sakurai et al., 1998).Radioligand binding studies (Van den Pol et al., 1998) indicated that orexin A has equal affinity for OX1 … Witryna开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 Witryna12 kwi 2024 · After resuspension in 250 µl of deionized water, 100 µl was used in duplicates to measure orexin-A immunoreactive peaks in RIA. An in-house orexin-A … maybury primary school morrison construction