Leptin assay
WebThis assay employs an antibody specific for Mouse Leptin coated on a 96- well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Leptin antibody is added. WebThe U-PLEX platform offers validated components that can be combined to create high quality assays quickly and easily. The U-PLEX Mouse Leptin Assay has the essential …
Leptin assay
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WebJan 9, 2013 · Abcam’s Leptin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Leptin concentrations in Human cell culture supernatants, serum and … WebLeptin is a 16-kilodalton protein that was first identified when it was cloned in 1994. 3,4 Humans and mice with defects in the gene that codes for leptin, referred to as the obese or OB gene, tend to become morbidly obese. 4 Obese mice with the ob/ob defect lose …
WebLeptin is a cytokine-like peptide secreted by adipocytes. It has a critical endocrine function, signalling to the hypothalamus the level of body fat reserves. Thus, in starvation, where … Web9 rows · This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research. Human Leptin "Dual …
WebMouse Leptin ELISA Kit (ab199082) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in cell culture supernatant, cit plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Leptin with 33.6 pg/ml sensitivity. WebThe Eagle Biosciences Functional Leptin ELISA Assay Kit is for the quantitative measurement of functional, receptor binding Leptin levels in serum or plasma. The kit is for research use only and not for diagnostic procedures. 2 INTRODUCTION Leptin a hormone of 146 amino acids shows a molecular weight of 16 kDa (P41159). It consists
WebThe product of the leptin gene is a 16-kDa protein secreted by adipose tissue and regulates adiposity. The leptin gene could be a potential candidate gene controlling some proportion of adipose and lean accretion in cattle, and thus, may be referred to as one of genetic factor controlling meat quality determinants such as marbling.
WebSep 6, 2012 · No significant differences were observed with these different protocols, except by using sonicated samples, where leptin concentration detected was lower; thus, we decided to use whole milk samples diluted 1:1 with the assay buffer. The recovery of added leptin using this protocol was 103.1 ± 1.4% (n = 4). Statistical Analysis pee proof mattress protectorWebPrinciple of Leptin ELISA Assay. The Leptin ELISA kit is a solid phase enzyme-linked immunosorbent assay based on the sandwich principle. The microtiter plates were pre-coated with monoclonal anti-leptin antibodies directed towards unique antigenic sites on leptin molecules. Standards and samples containing endogenous leptin were incubated … pee river crosswordWebFig.3 Leptin inhibition 3T3L1 adipocytes were plated in a 96-well plate at 50,000 cells/well/100 µl DMEM/FBS for 5 hours, and then deprived of serum for 1 hour with or without leptin (100 nM). Then add 10 nM insulin to the wells and incubate for another 30 minutes in a 37°C, 5% CO2 incubator. meaning telugu to englishWebMaternal serum leptin concentration was measured quantitatively by sandwich enzyme-linked immunosorbent assay technique using Leptin (human LEP) kit (Elabscience Biotechnology Co. Ltd, Wu Han, People’s Republic of China). The lowest limit of detection of the assay was 0.094 ng/mL. The intra-assay and interassay coefficients of variation ... pee remover carpetWebLeptin ELISA Assay is For Research Use Only Size: 1×96 wells Sensitivity: 0.5 ng/mL Dynamic Range: 1–100 ng/mL Incubation Time: 105 minutes Sample Type: Serum … meaning templateWebLeptin is a hormone that is primarily secreted by adipocytes and is involved in regulating hunger. Leptin mouse ELISA kit (ab100718) was used to test leptin levels in cell lysates of 3T3-L1 cells and adipocyte-like cells. As shown in Figure 2, leptin levels were increased in the cell lysates of adipocyte-like cells compared with 3T3-L1 cells. meaning tenaciousWebFeb 5, 2016 · Leptin resistance is considered to be the primary cause of obesity. However, the cause of leptin resistance remains incompletely understood, and there is currently no cure for the leptin-resistant state. In order to identify novel drug-target molecules that could overcome leptin resistance, it would be useful to develop in vitro assay systems for … meaning tentatively