WebFeb 1, 2024 · The CyTOF data used here (see Data description section) is already preprocessed; i.e., the normalization and de-barcoding, as well as removal of doublets, debris and dead cells, were already performed; further details are available in the Data preprocessing section. WebJul 23, 2024 · Mass cytometry time-of-flight (CyTOF) empowers us to understand acute myeloid leukemia (AML) biology at the single-cell level. This technology, combined with advanced data-analysis methods, enables identification and characterization of the rare AML stem/progenitor cells that play key roles in drug resistance and AML relapse.
Acquisition, Processing, and Quality Control of Mass ... - Springer
WebJul 13, 2024 · Bead-normalization is an approach introduced to mass cytometry as a pre-processing step to diminish the effect of such variations (Finck et al., 2013). Interestingly, application of DeepCyTOF to unnormalized and bead-normalized data did not show an advantage of using the latter for the task of automated gating. WebMay 22, 2015 · The normalizer tool embedded in the CyTOF software is a global normalizer, while the Matlab method focuses around a particular data set. Please refer to the user … global awards token
CyTOF workflow: differential discovery in high-throughput high ...
National Center for Biotechnology Information WebAug 19, 2014 · I know that bead-based normalization should theoretically allow us to normalize all CyTOF data across instruments, which would make data sets from … Webbead_slopes = compute_bead_slopes(bead_means, smoothing $ beads) # Checking that times are unique - in their code times are made unique (maybe this should be changed here) t = smoothing $ time global aware international