WebMar 1, 2024 · Circular RNA (circRNA) is a class of endogenous non-coding RNA characterized by a back-splice junction (BSJ). In general, large-scale circRNA BSJ …
Reconstruction of full-length circular RNAs enables …
WebFeb 28, 2024 · Performance comparison between CIRI and CIRI2. (A) Overlap of circRNA predictions by CIRI2 and CIRI on Hs68 data sets.(B) RNase R resistance of the CIRI and CIRI2 predictions for Hs68 data sets.(C) RNase R resistance and BSJ read count detected for each prediction by CIRI and CIRI2.Only predictions with BSJ read counts ≤150 are … WebFeb 7, 2024 · Therefore, all circRNAs identified by any one of the four BSJ-based algorithms were pooled, and a pseudo-reference for each candidate circRNA was constructed using sequence ± 149 bp flanking the BSJ sites (Fig. 1e) to overcome these problems. Reads that are directly aligned against the pseudo-reference should be derived explicitly from … cara memisahkan file word per bab online
Transcriptome-wide profiles of circular RNA and RNA-binding …
WebFeb 8, 2024 · The key challenge to the methods to detect circRNAs from RNA-seq data is identifying genuine back-splicing junction (BSJ) reads, which cover the linkage of the downstream-3’-splice-donor exon attached to the upstream-5’-splice-acceptor exon of a circRNA. The identification of a BSJ from RNA-Seq data does not always assure a true … WebJan 21, 2024 · Short hairpin RNAs (shRNAs) are used to deplete circRNAs by targeting back-splicing junction (BSJ) sites. However, frequent discrepancies exist between shRNA-mediated circRNA knockdown and the corresponding biological effect, querying their robustness. By leveraging CRISPR/Cas13d tool and optimizing the strategy for designing … WebMar 8, 2024 · Next, all ribo-circRNA BSJ sequences in one species were aligned to those of the other species using BLAT with default parameters , followed by a reciprocal best hit strategy to find the orthologous ribo-circRNAs. Finally, a pair of conserved ribo-circRNAs were defined based on their sequence alignment length ≥ 80 and alignment bit-score ≥ 150. cara memohon special pass imigresen online