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Ai9 tdtomato reporter mice

WebDec 20, 2009 · Strong tdTomato native fluorescence in Ai9 and Ai14 allowed direct visualization of fine structural details, such as dendritic spines and thin axons, of diverse … WebApr 6, 2024 · Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues.

Ai9 mouse SCGE Toolkit>

WebApr 10, 2024 · Ad5-knob(RGD/PK7) produce tdTomato signal in the hippocampus of Ai9 (loxP-tdTomato) mice. Fluorescence was abolished when the spike tip protein (Pvc10) was deleted, confirming that the observed activity was mediated by the ... For assays involving co-transfection of a Cre reporter plasmid or a guide. RNA plasmid, DNA was transfected … WebApr 11, 2024 · We therefore examined sections from brain and spinal cord of NPFF Cre mice crossed with the Ai9 reporter line, in which Cre-dependent excision of a STOP … movie bell book and candle cast https://tywrites.com

Generation of a MyoD knock-in reporter mouse line to study …

WebPlasmid pDEST-Cas9-tdTomato from Dr. Hideyuki Okano's lab contains the insert Cas9 and is published in PLoS One. 2024 Aug 27;14(8):e0221164. doi: … WebJan 1, 2024 · Here, we examined the distribution of Cre and UCP1 throughout the body in UCP1-Cre reporter mice. Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues. WebDec 27, 2024 · Given that low transduction efficiency of these serotypes by intravitreal injection 19, we delivered them to the retinas of Ai9 mice by subretinal injection (Fig. 1 A). We observed distinct... movie bell book and candle

007909 - Ai9 or Ai9(RCL-tdT) Strain Details

Category:Ai9 mouse (BCM) SCGE Toolkit>

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Ai9 tdtomato reporter mice

A robust and high-throughput Cre reporting and ... - Nature

WebNov 12, 2024 · C57BL/6 (wild type, WT) and Ai14/Rosa26-tdTomato Cre-reporter (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) male and female mice (6–8 weeks old) … WebThis was achieved by crossing the HDC-Cre mouse line with Rosa26-tdTomato reporter mice, thus resulting in the expression of the fluorescent Tomato (Tmt) signal in cells …

Ai9 tdtomato reporter mice

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WebThe Jackson Laboratory tdtomato cre reporter ai9 mouse line Tdtomato Cre Reporter Ai9 Mouse Line, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebDec 13, 2024 · Ai9 Cre Reporter Mouse Strain and Study Plans for AAV Local and Systemic Delivery (A) Ai9 Cre reporter mouse strain with tdTomato expression induced by Cre recombinase expressed by a viral vector, which excises the stop cassette upstream of the tdTomato expression cassette.

WebApr 8, 2024 · Myoblast determination protein 1 (MyoD) dynamics define the activation status of muscle stem cells (MuSCs), aiding in muscle tissue regeneration after injury. However, the lack of experimental platforms to monitor MyoD dynamics in vitro and in vivo has hampered the investigation of fate determination and heterogeneity of MuSCs. Herein, … WebAug 12, 2024 · Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt (ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination.

WebAi9 mouse (BCM) Official Name: B6.Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J: Species: Mouse: Type: Animal: Description: Ai9 mouse has a loxP-flanked STOP … WebHere, we present a novel reporter mouse model targeting a microglia-specific marker, TMEM119, for studying microglia in health and disease. By placing a reporter cassette (GSG-3xFlag-P2A-tdTomato) between the coding sequence of exon 2 and 3'UTR of the Tmem119 gene using CRISPR/Cas9 technology, we generated a Tmem119-tdTomato …

Web用于例如CNS、PNS、内耳、心脏、或视网膜中的转基因表达的工程化AAV载体及其使用方法。还提供了开发在期望的细胞类型中介导转基因表达的新工程化AAV载体的方法。

WebThe Jackson Laboratory αsmacreert2 ai9 col2 3 gfp αsmacreert2 Ai9 Col2 3 Gfp, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more movie below her mouthWebThe ratio of the reporter to the reporter control in ESCC mice was also significantly higher than in healthy control mice (P<0.0001; Figure 5C and G). Moreover, the ROC curve … heather dyer southern pines ncWebJan 5, 2024 · The Ai9 reporter mice harbors a lox-STOP-lox (LSL)-tdTomato reporter knockin at the Rosa26 locus 11 (Figure 1). Using two sgRNAs flanking LSL, Cas9 will delete the STOP cassette by NHEJ, resulting in tdTomato expression. 12 This system allows direct detection of cells edited by CRISPR in vivo. movie bell book and candle free onlineWebSep 11, 2024 · Tamoxifen-inducible Osx-Cre-ERT2;Ai9/TdTomato reporter mice (male and female) were aged to young adult (5 months) and middle age (12 months), and were … movie bell book candleWebDec 31, 2015 · As expected, injection of AAV- Dmd CRISPR did not induce tdTomato expression in satellite cells or myofibers of mdx;Ai9 mice (fig. S11). In summary, this study provides proof-of-concept evidence supporting the efficacy of in vivo genome editing to correct disruptive mutations in DMD in a relevant dystrophic mouse model. heather dykstraWeb口腔医学小站 口腔文献阅读与资源分享. 2024年4月3日 发表者 kimi1006 暂无评论. Programmable protein delivery with a bacterial contractile injection system Nature heather dysonWeb007909 B6.Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG … movie belle starr with elizabeth montgomery